The spike gene target failure (SGTF) genomic signature is highly accurate for the identification of Alpha and Omicron SARS-CoV-2 variantsArticle Published on 2022-11-082022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 유전자 메커니즘, 치료기술, 치료제, [키워드] 95% CI Accuracy Alpha variant Amino acid Analysis B.1.1.529 B.1.1.7 cause Del detect evaluated genomic Laboratory Mutation omicron positive Positive predictive value Protein resulting RT-PCR S gene SARS-CoV-2 SARS-CoV-2 RT-PCR SARS-CoV-2 variant Sensitivity and specificity sequenced Sequencing spike gene TaqPath tested the Spike Thermo Fisher variant variants of concern VoC [DOI] 10.1038/s41598-022-21564-y PMC 바로가기
The mutation features and geographical distributions of the surface glycoprotein (S gene) in SARS-CoV-2 strains: A comparative analysis of the early and current strainsArticle Published on 2022-11-012022-11-16 Journal: Journal of medical virology [Category] SARS, 변종, [키워드] ACE2-binding region acute respiratory syndrome Analysis analyzed coronavirus coronavirus disease COVID-19 COVID-19 vaccines demonstrated develop Evolution feature Frequency Genome genome mutations geographical distribution highest identify loci mutant strain Mutation omicron prevalent remained Result S gene S protein SARS-CoV-2 SARS-CoV-2 strain SARS-CoV-2 variant strain Strains Surface glycoprotein Surface glycoprotein. the receptor-binding domain the S protein The United States United Kingdom Vaccines was used [DOI] 10.1002/jmv.28023 PMC 바로가기
Automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) and lateral flow strip based on CRISPR/Cas13a for sensitive and visual detection of SARS-CoV-2SARS-CoV-2의 감도 및 시각적 검출을 위한 CRISPR/Cas13a 기반의 자동화된 휴대용 고처리량 형광 분석기(APHF 분석기) 및 측면 흐름 스트립Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed amplification automated biosensor Care Contamination coronavirus COVID-19 CRISPR CRISPR/Cas13a detect Detection limit develop globe Health Lateral lateral flow strip MOST On-site detection ORF1ab gene POCT Point point of care Point of care testing (POCT) polymerase provided recombinase polymerase amplification Recombinase polymerase amplification (RPA) RPA S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. severe acute respiratory syndrome Coronavirus specificity Visualization [DOI] 10.1016/j.talanta.2022.123594 PMC 바로가기 [Article Type] Article
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Comparison of viral propagation and drug response among SARS-CoV-2 VOCs using replicons capable of recapitulating virion assembly and releaseArticle Published on 2022-10-012022-11-15 Journal: Virologica Sinica [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome assays Beta changed coronavirus Delta delta VoCs drug resistance drug response ENhance Evolution of SARS-CoV-2 genomes immune evasion inhibitor manifested Mutation novel antiviral drugs Protein provide quantification released remained Remdesivir replicon RNA S gene SARS-CoV-2 sgRNA supernatant Support Transcription variants variants of concern variants of concern (VOC) viral genome replication viral infectivity viral life cycle viral propagation Viral protein Viral-like particle (VLP). viral-like particles virion assembly VLP VoC VOCs WIV04 strain [DOI] 10.1016/j.virs.2022.06.008 PMC 바로가기
Immediate reinfection with Omicron variant after clearance of a previous SARS-CoV-2 infectionArticle Published on 2022-09-012022-11-16 Journal: Journal of infection and public health [Category] COVID19(2023년), SARS, 변종, [키워드] Alpha variant clearance Combination COVID-19 Delta epidemic peak heterogeneity Immediate immune evasion individuals Infection Italy Lineage Microbiology omicron Patient Primary infection Regional Reinfection RT-PCR test S gene SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2. searched Sequencing Transmissibility variant variants whole genome [DOI] 10.1016/j.jiph.2022.07.013 PMC 바로가기
Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2SARS-CoV-2 순환 변이체: Delta, Omicron BA.1 및 BA.2를 신속하게 스크리닝하기 위한 indel 돌연변이에 기반한 간단한 유전형 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Analytical specificity circulating circulating SARS-CoV-2 variants concern Concordance COVID-19 case Delta Delta VOC develop development facilitate flexible genotyping identify implementation melting curve analysis Mutation mutations omicron Partial partial sequencing performed primer public health qPCR assay qPCR assays S gene SARS-CoV-2 Screening screening method Screening. Sequencing verification VOCs [DOI] 10.1016/j.jviromet.2022.114570 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Brain Regional Detection, Histopathology, Gene Expression, and Immunomodulatory Changes in Decedents with COVID-19코로나19 사망자의 SARS-CoV-2 뇌 부위 검출, 조직병리학, 유전자 발현 및 면역조절 변화Article Published on 2022-08-162022-09-11 Journal: Journal of neuropathology and experimental neurolo [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] Amygdala arising Autopsies Autopsy Blood Brain brains cerebellar cortex change Control COVID-19 COVID-19 cases cytokine Deafferentation detection Differentially expressed genes downregulation Encephalitis fraction Gene Expression Genes Hemorrhagic hemorrhagic encephalitis Histopathology immune immune response indicated involved marker microglia mucosa neuronal Non-COVID-19 olfactory olfactory bulb portal receptor reduced reduction Region Regional RNA polymerase RNA sequence RNA sequences RNA-dependent RNA polymerase RNAseq RT-PCR S gene SARS-CoV-2 SARS-CoV-2 E gene SARS-CoV-2. sequence serum Significant subject upregulation viral sequence virus with COVID-19 [DOI] 10.1093/jnen/nlac056 PMC 바로가기 [Article Type] Article
Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design230,163 SARS-CoV-2 게놈에서 보존 영역 식별 및 진단 PCR 프라이머 디자인에서의 사용Article Published on 2022-08-012022-09-11 Journal: Genes & genomics [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition Alpha amplification approach Beta bioinformatics can be used candidate Candidates characteristic conserved Coverage dataset Delta Diagnosis diagnostic Epsilon Eta evaluated exhibited False negative full-length Gamma genome sequence genomes genomic sequence GISAID GISAID EpiCoV database identification identify in silico Intensive Iota Multiple Multiple sequence alignment multiple sequence alignments NCBI nine NSP2 orf3a gene Outbreaks pandemic PCR PCR primer primer primer set primer sets Primers qPCR RdRP Region removal retrieved RNA sample RNA samples RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 variant SARS-CoV-2. sequence Sequence alignment target viral genome viral sequences was reduced Zeta [DOI] 10.1007/s13258-022-01264-7 PMC 바로가기 [Article Type] Article
The short-term effect of different chlorhexidine forms versus povidone iodine mouth rinse in minimizing the oral SARS-CoV-2 viral load: An open label randomized controlled clinical trial studyRandomized Controlled Trial Published on 2022-07-292022-10-05 Journal: Medicine [Category] COVID19(2023년), SARS, 임상, [키워드] ARMS assigned baseline collected conducted control group Controlled clinical trial COVID-19 E gene Efficiency evaluated form four group group Intervention less male open label P-value participant Patient povidone randomization Randomized real time PCR recruited reduction S gene Saliva salivary SARS-CoV-2 SARS-CoV-2 viral second significant decrease statistically significant difference subject the SARS-CoV-2 threshold Viral load was collected was recorded [DOI] 10.1097/MD.0000000000028925 PMC 바로가기 [Article Type] Randomized Controlled Trial